Multiplexed CRISPR-Cas9-Based Genome Editing of Rhodosporidium toruloides
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چکیده
منابع مشابه
Efficient Mitochondrial Genome Editing by CRISPR/Cas9
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has been widely used for nuclear DNA editing to generate mutations or correct specific disease alleles. Despite its flexible application, it has not been determined if CRISPR/Cas9, originally identified as a bacterial defense system against virus, can be targeted to mitochondria for mtDNA editing. Here, we show t...
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The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has ...
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Background: CRISPR/Cas9-based genome editing holds a great promise to accelerate the development of improved crop varieties by providing a powerful tool to modify genomic regions controlling major agronomic traits. The effective deployment of this technology for crop improvement still requires further optimization of the CRISPR/Cas9 system for different applications. Here we have optimized mult...
متن کاملTitle: Optimizing multiplex CRISPR/Cas9-based genome editing for wheat
not peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was . http://dx.doi.org/10.1101/051342 doi: bioRxiv preprint first posted online May. 2, 2016;
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ژورنال
عنوان ژورنال: mSphere
سال: 2019
ISSN: 2379-5042
DOI: 10.1128/msphere.00099-19